Biotechnology Principles And Processes Chapter-Wise Test 1

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following statements about Southern blotting is correct?

Southern blotting is used to detect specific DNA sequences.
Southern blotting is used to detect proteins.
Southern blotting does not require gel electrophoresis.
Southern blotting is not useful in genetic analysis.
1

Southern blotting is a molecular biology technique used to detect specific DNA sequences in a sample.

Which of the following statements about DNA ligase is correct?

DNA ligase is used to join DNA fragments by forming phosphodiester bonds.
DNA ligase is responsible for cutting DNA at specific recognition sites.
DNA ligase is not required in recombinant DNA technology.
DNA ligase prevents DNA replication.
1

DNA ligase forms phosphodiester bonds between DNA fragments, sealing nicks in the sugar-phosphate backbone.

Which of the following is used as a selectable marker in a plasmid vector?

Origin of replication
Antibiotic resistance gene
Recognition sequence
Restriction enzyme
2

Selectable markers, such as antibiotic resistance genes, help in identifying recombinant cells.

Which of the following is used as a vector in the production of genetically modified plants?

Ti plasmid
Lambda phage
Taq polymerase
Ligase
1

The Ti plasmid of Agrobacterium tumefaciens is commonly used as a vector for gene transfer in plants.

Which method is used to introduce foreign DNA into bacterial cells?

Southern blotting
Microinjection
Heat shock transformation
Gel electrophoresis
3

Heat shock transformation increases bacterial membrane permeability, allowing DNA uptake.

Which of the following is NOT a characteristic of a cloning vector?

Origin of replication
Selectable marker
Multiple restriction sites
Inability to replicate
4

Cloning vectors must be able to replicate within a host cell to ensure the propagation of the inserted gene.

Which of the following statements about genetically modified organisms (GMOs) is incorrect?

GMOs can be used to produce pharmaceuticals.
GMOs can be engineered for pest-resistant crops.
GMOs have no applications in medicine.
GMOs can be created using recombinant DNA technology.
3

GMOs have various medical applications, including the production of insulin, vaccines, and other therapeutic proteins.

Which of the following best describes gel electrophoresis?

A technique to separate DNA fragments by size
A method to amplify DNA sequences
A technique to insert genes into cells
A process for synthesizing proteins
1

Gel electrophoresis is used to separate DNA fragments based on size using an electric field.

In recombinant DNA technology, the function of restriction enzymes is to:

Join DNA fragments
Cut DNA at specific sites
Synthesize mRNA
Unwind the DNA helix
2

Restriction enzymes cut DNA at specific sequences, generating sticky or blunt ends for recombination.

Which of the following statements about Southern blotting is incorrect?

Southern blotting is used to detect specific DNA sequences.
Southern blotting does not require gel electrophoresis.
Southern blotting involves DNA hybridization.
Southern blotting is useful in forensic science.
2

Southern blotting requires gel electrophoresis to separate DNA fragments before hybridization.

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